LOCUS       Exported                5016 bp DNA     circular SYN 12-MAR-2024
DEFINITION  Shuttle vector pSET2 DNA, complete sequence.
ACCESSION   AB042430
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5016)
  AUTHORS   Takamatsu D, Osaki M, Sekizaki T.
  TITLE     Construction and characterization of Streptococcus suis-Escherichia 
            coli shuttle cloning vectors
  JOURNAL   Plasmid 45 (2), 101-113 (2001)
  PUBMED    11322824
REFERENCE   2  (bases 1 to 5016)
  AUTHORS   Takamatsu D, Osaki M, Sekizaki T.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-MAY-2000) Daisuke Takamatsu, National Institute of 
            Animal Health, Laboratory of Molecular Bacteriology; Kannondai 
            3-1-1, Tukuba, Ibaraki 305-0856, Japan 
            (E-mail:p1013dt@niah.affrc.go.jp, Tel:81-298-38-7743, 
            Fax:81-298-38-7743)
REFERENCE   3  (bases 1 to 5016)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plasmid"; 
            date: "2001"; volume: "45"; issue: "2"; pages: "101-113"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (08-MAY-2000) Daisuke Takamatsu, National Institute of Animal
            Health, Laboratory of Molecular Bacteriology"; pages: 
            "81-298-38-7743"
FEATURES             Location/Qualifiers
     source          1..5016
                     /lab_host="Streptococcus suis, Escherichia coli"
                     /mol_type="other DNA"
                     /label=synonym:Shuttle Cloning Vector pSET2
                     /note="synonym:Shuttle Cloning Vector pSET2"
                     /db_xref="taxon:125568"
                     /organism="Shuttle vector pSET2"
     gene            156..176
                     /gene="dso"
                     /label=dso
     misc_feature    156..176
                     /gene="dso"
                     /label=double-stranded replication origin
                     /note="double-stranded replication origin"
     gene            367..504
                     /gene="copG"
                     /label=copG
     CDS             367..504
                     /codon_start=1
                     /gene="copG"
                     /product="CopG, transcriptional repressor protein"
                     /function="transcriptional repressor protein"
                     /label=copG
                     /protein_id="BAB03239.1"
                     /translation="MKKRLTITLSESVLENLEKMAKEMGLSKSALISVALENYKKGQVK
                     "
     gene            566..1246
                     /gene="repB"
                     /label=repB
     CDS             566..1246
                     /codon_start=1
                     /gene="repB"
                     /product="RepB, replication initiation¨Ctermination
                     protein."
                     /function="The plasmid-encoded initiator of replication.The
                     protein introduces a strand- and site-specific nick in the 
                     double-stranded replication origin, on supercoiled DNA."
                     /label=repB
                     /protein_id="BAB03240.1"
                     /translation="MAKEKARYFTFLLYPESIPSDWELKLELLGVPIAVSPLHDRDKSD
                     VEGQQYKKPHYHVIYVSKNPVTADSVRMKIKRSLGDNSVALVQIIRTSIENTYLYLTHE
                     SKDAIEKKKHVYDKADITLLSNFDIDRYITLDVEEKDDMLNEVCDLIDEYDIANMRELR
                     RFIKLHGAEHGLPSIKIINSVLRSHTGLIRLYFDAVYQERKYGSNIPNVNQETGEILSE
                     DSDD"
     gene            complement(1703..2470)
                     /gene="add9"
                     /label=add9
     CDS             complement(1703..2470)
                     /codon_start=1
                     /gene="add9"
                     /product="spectinomycin adenyltransferase, aso known as
                     aad9."
                     /function="spectinomycin resistance"
                     /label=spc
                     /protein_id="BAB03241.1"
                     /translation="MRRIYLNTYEQINKVKKILRKHLKNNLIGTYMFGSGVESGLKPNS
                     DLDFLVVVSEPLTDQSKEILIQKIRPISKKIGDKSNLRYIELTIIIQQEMVPWNHPPKQ
                     EFIYGEWLQELYEQGYIPQKELNSDLTIMLYQAKRKNKRIYGNYDLEELLPDIPFSDVR
                     RAIMDSSEELIDNYQDDETNSILTLCRMILTMDTGKIIPKDIAGNAVAESSPLEHRERI
                     LLAVRSYLGENIEWTNENVNLTINYLNNRLKKL"
     gene            complement(2872..3195)
                     /gene="lacZ'"
                     /label=lacZ'
     CDS             complement(2872..3195)
                     /codon_start=1
                     /gene="lacZ'"
                     /product="beta-galactosidase alpha fragment"
                     /label=lacZ'
                     /protein_id="BAB03242.1"
                     /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICS
                     DAA"
     primer_bind     3105..3121
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    3122..3178
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(3191..3207)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3215..3231
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3239..3269)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3284..3305
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     gene            4111..4252
                     /gene="ssoA"
                     /label=ssoA
     misc_feature    4111..4252
                     /gene="ssoA"
                     /label=single-stranded replication origin
                     /note="single-stranded replication origin"
ORIGIN
        1 attaattttg aaatattaaa tatttttgga tatataggtg ttaagtttga aattattacc
       61 gcttatgaaa atgggaaatt agttgaaacg caaattattg tggatgacat agagttgata
      121 aaagttgaaa aattttttga ttaaagagaa gaagaggggg cgtactacga cccccctttt
      181 aagtgccgag tgccaaattg ctaaaaaaag tgccgtaaag ctagatacat caagggttta
      241 gctcatgcta cacttcggcg acattttggc gacattttcg gcgacaaaaa gaaaaaaata
      301 cgataaatgt tgcaatgtat gcacagttat gctactatgg ttttataaat ttttgagagg
      361 tggaacatga aaaaaagatt gacgataaca ctcagtgaat cggtgcttga aaatcttgaa
      421 aaaatggcaa aagagatggg gttgtcaaaa tctgcactga tttcagttgc cttggaaaac
      481 tacaagaaag gtcaagtaaa ataaaaaaaa gccgagctgg caggcgggct tttaaaagta
      541 ttataacttg ggttaattat aacttatggc taaagaaaaa gcaagatact tcactttttt
      601 actctatcct gagtcaatcc ctagtgattg ggaattgaaa ttagaattgc tgggcgtacc
      661 tattgcagtc agccctttgc atgatagaga caagagtgat gttgagggac aacaatataa
      721 aaaaccgcac tatcatgtga tttatgtttc aaaaaatcca gtcacggcag atagtgtgag
      781 aatgaaaatt aagaggtcac ttggagataa tagcgttgct ctagttcaga taattagaac
      841 tagcattgag aacacgtatt tatatcttac gcatgaatca aaggacgcta tcgaaaagaa
      901 aaagcacgtc tatgataagg ctgacataac tttgcttagt aactttgata tagaccgtta
      961 tataacgctt gacgtcgaag agaaagacga tatgctcaat gaggtttgtg accttattga
     1021 tgagtatgat attgcaaata tgcgtgaatt gagacgtttt attaaacttc atggggctga
     1081 acatggttta cctagtatca aaattataaa ttcagtttta cgttcgcata ctgggttaat
     1141 acgtctgtat tttgatgctg tctatcaaga acgtaagtat ggcagtaata tccctaatgt
     1201 taatcaagag acaggcgaga ttttaagtga ggatagcgat gactgataga aatgagctga
     1261 ttaacgatat tgcagagttg aaagcaaaaa gagacagact actggctcaa atgaaagagg
     1321 cggaacaatg ggaaagcgta gcgtgggata gttactatgc agtcgcagac catgtgaaag
     1381 ctcttgagaa aaagcaggag ataggtcgaa actattggga aagttctcaa agagctatta
     1441 gtcatcaatt tgactttgtg gctgaccaag ctaacaaagt caaaaaggtg ttggctaaaa
     1501 agagatatga tttgctagat gaggaaatcg acaagctgat gaacgaagtt cgagagctgg
     1561 cagatgtcct tgggattgag attgacgagc tcggtttatt gttttctaaa atctgattac
     1621 caattagaat gaatatttcc caaatattaa ataataaaac aaaaaaattg aaaaaagtgt
     1681 ttccaccatt ttttcaattt ttttataatt tttttaatct gttatttaaa tagtttatag
     1741 ttaaatttac attttcatta gtccattcaa tattctctcc aagataacta cgaactgcta
     1801 acaaaattct ctccctatgt tctaatggag aagattcagc cactgcattt cccgcaatat
     1861 cttttggtat gattttaccc gtgtccatag ttaaaatcat acggcataaa gttaatatag
     1921 agttggtttc atcatcctga taattatcta ttaattcctc tgacgaatcc ataatggctc
     1981 ttctcacatc agaaaatgga atatcaggta gtaattcctc taagtcataa tttccgtata
     2041 ttcttttatt ttttcgtttt gcttggtaaa gcattatggt taaatctgaa tttaattcct
     2101 tctgaggaat gtatccttgt tcataaagct cttgtaacca ttctccataa ataaattctt
     2161 gtttgggagg atgattccac ggtaccattt cttgctgaat aataattgtt aattcaatat
     2221 atcgtaagtt gcttttatct cctatttttt ttgaaatagg tctaattttt tgtataagta
     2281 tttctttact ttgatctgtc aatggttcag atacgacgac taaaaagtca agatcactat
     2341 ttggttttag tccactctca actcctgatc caaacatgta agtaccaata aggttatttt
     2401 ttaaatgttt ccgaagtatt tttttcactt tattaatttg ttcgtatgta ttcaaatata
     2461 tcctcctcac tattttgatt agtacctatt ttatatccat agttgttaat taaataaact
     2521 taatttagtt tatttataga tttcattggc ttctaaattt tttatctaga taataattat
     2581 tttagttaat tttattctag attatatatg atatgatctt tcatttccat aaaactaaag
     2641 taagtgtaaa cctattcatt gttttaaaaa tatctcttgc cagtcacgtt acgttattag
     2701 ttatagttat tataacatgt attcacgaac agatctcggt gatgacggtg aaaacctctg
     2761 acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca
     2821 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc
     2881 atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt
     2941 aaggagaaaa taccgcatca ggcgccattc gccattcagg ctgcgcaact gttgggaagg
     3001 gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag
     3061 gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag
     3121 tgaattcgag ctcggtaccc ggggatcctc tagagtcgac ctgcaggcat gcaagcttgg
     3181 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     3241 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     3301 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     3361 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     3421 cctcgctcac tagaaagccc ctagaagacg aaataaggac tttgaatgaa gttcttaagg
     3481 aaaaatacga ggagttagac aaaatcgagc tacgagcctc tgagagcctc tcagagctat
     3541 ctggtgtcga agggtatata aataacctag aaaaacattc aagcgcctta gaaagcaaaa
     3601 tagggggctt agaaaacgaa tatttaagac tggctaaaca aaatgcccga ttaagcgact
     3661 tgaaaatcat gtctgaaaaa gaattggctg aaattcagcc gaaaaaaggc atgtttggaa
     3721 aagaacatgt cgaattgact aaagagcaat ttgagaaatt taagggattg atataccgaa
     3781 gcaagaattt agttcatgaa aaggaattag agaatgacca actaaggcgg caaatgcctc
     3841 tgagaagctc aaaaaacggt tttgaggcga gtttgcaacg ggctaaggat aaaactaaag
     3901 gagaaagcat agaccgcctt aaaagcgaaa ttagagggct taaaaatgaa aactcggttt
     3961 tgagacagca aaatgacaaa atgctaggta agttaagaga gtttatgcct gataaagcgt
     4021 taaataattt tatttctgaa cttaaatcaa taaaacctat cgtgaagata gttaaaagag
     4081 ttattgaaaa aggcttaggg ctctaagcga tttatgccga gaaaactctt gctaggaagc
     4141 tatgcgaaat agactagtag gggtcgagtc gacaggctga aagcttgccg accgaacacg
     4201 acagtcagat ttcagcatag gtttcttcct agcaagagga aattggaata aagaaagcgt
     4261 atcaaagtaa aactaaaagg actaagtcct gtgtaataca ggccttagtc ctttacttaa
     4321 aatagaatgt ccaacttttt ggggtcagta tatattttag tcttttttat tctatagttg
     4381 ggatagagat ttgtactcct cctgcgccat atgcaattcc gcttatacct gcatttcttg
     4441 cttttttagg taaaccgaat gaaccactaa ctgattgacc tcctttgcca gatttgctga
     4501 caggatatat tctagtagtg ttatctgaga aagtaactgt tatcaatcca gaatatgtat
     4561 aactttttga tgtattaggt ttaactgacc accaaacttc tcgaccacca ccatataatt
     4621 cgatttttgt atatccgcca tttccgtaac ttgttttgta aggaactatt tcaccggtat
     4681 aagaataatc agttgtagtt gaaatatttg tatttgaggt agtgactgta ttatctgcaa
     4741 aaacagtaga aatggatata aatcctaaca tagaaaaaag agtgacaaat acaattattg
     4801 attttttgaa acttttcatg aaagtttacc tccgtttttt tgttataatt atagcaaaaa
     4861 aataattaaa aagaaagcgt tatcatagaa atgaaaaaga attttattgc aattataaaa
     4921 aacatattaa aaaacaaaaa tatccctaaa tattgttttg aaattattga aaaagatgga
     4981 agtacttgtt ttctttcgac aaattacttt gatgta
//
